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Smallpox (Variola Virus), Pediatric


Basics


Description


  • Smallpox is a life-threatening, acute, eruptive, contagious disease caused by variola virus.
  • The disease is characterized by a febrile prodrome followed by the development of rash.
  • Rash evolves in a characteristic fashion: macules ’ † ’ papules ’ † ’ vesicles ’ † ’ pustules; scabs form and fall off, leaving scars called pockmarks.
  • There are 2 clinical forms of smallpox:
    • Variola minor is a less common and less severe form of disease.
    • There are 5 types of variola major, the more common and serious form of disease.
      • Ordinary smallpox
      • Modified smallpox
      • Flat smallpox
      • Hemorrhagic smallpox
      • Variola sine eruptione

Epidemiology


  • The last documented case of endemic smallpox was in Somalia in 1977.
  • The last case in the United States was in the late 1940s.
  • Smallpox was declared eradicated by the World Health Organization in 1979.
  • Historically in unvaccinated individuals, ordinary smallpox accounted for 90% of cases, hemorrhagic smallpox for 7% of cases, and flat and modified smallpox for the remainder.
  • Modified smallpox was rare in unvaccinated individuals but accounted for 25% of cases of disease in vaccinated individuals.

General Prevention


  • Prior to 1972, all U.S. children were vaccinated.
  • Vaccines were produced from the vaccinia virus, a closely related orthopoxvirus to variola.
  • Historically, the vaccine was prepared from virus grown on the skin of animals, and in some cases, the vaccine was contaminated with animal proteins, bacteria, and other viruses.
  • Newer smallpox vaccines are developed from vaccinia clones grown in tissue culture and therefore are free of contamination from bacteria and other viruses.
  • Only laboratories in the United States and Russia currently have stockpiles of smallpox virus.
  • Due to concern for use of smallpox as an agent of bioterrorism, the U.S. Strategic National Stockpile still stores smallpox vaccine.
  • The only currently FDA-licensed smallpox vaccine, ACAM2000 (which replaced Dryvax), is used for active immunization of persons determined to be at highest risk for infection.
  • The Advisory Committee on Immunization Practices recommends smallpox vaccination for the following:
    • Public health response teams responsible for investigating suspected smallpox cases
    • Hospital-based health care teams responsible for assessing and caring for suspected smallpox cases
  • Vaccine efficacy
    • 95% efficacious in preventing disease if given prior to exposure
    • May prevent smallpox or decrease severity if given 1 " “3 days after exposure
    • May decrease severity of disease if given 4 " “7 days after exposure
  • Vaccination is estimated to provide protective immunity for 3 " “10 years but may decrease the severity of disease for 10 " “20 years.
  • Vaccine administration
    • A skin abrasion is created using a bifurcated needle dipped in vaccine.
    • The vaccine site should be loosely covered to prevent the spread of virus to others.
    • After 3 " “4 days, a red pruritic papule appears at the vaccination site, which evolves into a vesicle followed by a pustule; after a few weeks, a scab forms, then falls off leaving a scar.
  • Contraindications to vaccine:
    • Atopic dermatitis or exfoliative skin disorder
    • Immunosuppression
    • Pregnancy or breastfeeding
    • Close contact of someone who is pregnant, immunosuppressed, or has skin disease
    • Allergy to vaccine component
    • Moderate or severe acute illness
    • Inflammatory eye disease
    • Heart disease (myocardial infarction, stroke, cardiomyopathy, heart failure, or angina) or ≥3 risk factors for heart disease
    • Age <1 year
    • These contraindications may be reevaluated if smallpox is reintroduced into the population.
  • Common adverse reactions to vaccination:
    • Fever, swelling, lymphadenitis, and headache are seen in 2 " “16% of adults receiving the vaccine for the first time.
    • A mild rash occurs in ’ ˆ Ό8% of cases.
  • Less common vaccine reactions:
    • Vaccinia keratitis and/or vision loss
    • Accidental inoculation with blister formation
    • Moderate to severe generalized rash
    • Eczema vaccinatum
    • Encephalitis
    • Congenital or generalized vaccinia
    • Myopericarditis
    • Progressive vaccinia/vaccinia gangrenosum
    • Bacterial superinfection

Pathophysiology


  • The virus infects the upper respiratory tract and replicates; rarely, primary infections can occur via skin, conjunctival, or placental routes.
  • The virus enters the bloodstream (primary viremia) and is taken up by macrophages.
    • Patient is asymptomatic during this time.
  • Next, the virus enters the reticuloendothelial system where it continues to replicate.
  • Secondary viremia occurs as the virus reenters the bloodstream and infects organs.
    • Can cause epidermal necrosis and swelling
    • Infections of the bone marrow, kidneys, liver, lymph nodes, spleen, and other organs result in coagulopathy and multiorgan system failure.
  • Exact mechanisms of viral toxicity are not understood but may involve both viral cytopathic effects and inflammatory pathology.

Etiology


  • Variola virus is a member of the poxvirus family and (Orthopox genus).
  • Variola is a double-stranded DNA virus most commonly transmitted during face-to-face contact via respiratory aerosols or direct contact with infected skin lesions.
  • Transmission of the virus via air in enclosed settings or via infected fomites is uncommon.
  • Humans are the only vectors.

Diagnosis


  • Ordinary smallpox
    • Incubation period lasts 7 " “17 days, followed by a 1 " “4 day febrile prodrome characterized by high fever, headache, back pain, chills, abdominal pain, and emesis.
    • Eruptive phase begins with lesions of the mouth, tongue, and oropharynx.
    • The rash
      • Often starts on face and spreads to rest of body within 24 " “48 hours
      • On day 1, rash is macular.
      • On day 2, rash becomes papular.
      • On days 4 and 5, rash becomes vesicular.
      • By day 7, rash becomes pustular.
      • By 2 " “3 weeks, scabs form.
      • Scabs fall off and leave pockmarks.
  • Modified smallpox
    • Milder than ordinary smallpox
    • Accelerated course
    • Lesions are not as deep
  • Flat smallpox
    • Characterized by soft, flat, semiconfluent or confluent rash that does not evolve to pustules but can still result in significant skin loss
  • Hemorrhagic smallpox
    • Shorter incubation time
    • Skin becomes dusky
    • Bleeding in skin and mucous membranes
    • Can be difficult to diagnose unless exposure to variola virus is known
  • Variola sine eruptione
    • May be asymptomatic or cause an influenza-like illness
    • Noncontagious
    • Seen in infants with protective maternal antibodies and in vaccinated individuals
  • If there has not been a release or circulation of smallpox, the CDC protocol for evaluating patients for smallpox can be used to guide the assessment of a suspicious rash illness.
  • The CDC risk evaluation tool can be found at http://www.bt.cdc.gov/agent/smallpox/diagnosis/riskalgorithm/
    • If a patient has an acute, generalized rash on the body with vesicles or pustules, use the major and minor criteria to assess the likelihood of smallpox.
    • Major criteria
      • Febrile prodrome: 1 " “4 days prior to rash onset, including a temperature ≥101 ‚ °F and 1 or more of the following: prostration, headache, backache, chills, vomiting, or severe abdominal pain
      • Classic smallpox lesions are deep-seated, firm/hard, round, well-circumscribed vesicles or pustules that can become umbilicated or confluent as they evolve on any one part of the body (e.g., the face or arm); all the lesions are in the same stage of development.
    • Minor criteria
      • Centrifugal distribution with greatest concentration of lesions on face and extremities
      • First lesions appear on the oral mucosa, palate, face, or forearms.
      • Patient appears toxic or moribund
      • Slow evolution: Lesions evolve from macules to papules to pustules over days (each stage lasts 1 " “2 days).
      • Lesions on the palms and soles
    • High risk of smallpox
      • Febrile prodrome and classic lesions in same stage of development
    • Moderate risk of smallpox
      • Febrile prodrome and either 1 other major criterion or ≥4 minor criteria
    • Low risk of smallpox
      • No febrile prodrome, or febrile prodrome and <4 minor criteria

Diagnostic Tests & Interpretation


Diagnostic Procedures/Other
  • Use the CDC smallpox evaluation protocol to guide testing.
    • If high risk of smallpox:
      • Consult infectious disease and/or dermatology.
      • Public health agency will advise on management and collection of samples.
      • Testing will be performed at an approved laboratory prior to other tests.
    • If moderate risk of smallpox:
      • Consult infectious disease and/or dermatology.
      • Perform testing for varicella and other disorders including herpes simplex virus as indicated.
      • If no diagnosis is made after testing, ensure adequacy of specimen and have consultants reevaluate.
      • If smallpox still cannot be ruled out, then classify case as high-risk case.
    • If low risk of smallpox, and history and physical exam are highly suggestive of varicella, then varicella testing is optional.
    • If low risk of smallpox and diagnosis is uncertain, then testing should be done for varicella and other disorders as indicated.
  • Variola testing
    • Should not be performed in low- and moderate-risk cases because of risk of false positives
    • Should only be performed in designated high-containment facilities
    • Lesion specimens (fluid, cells, and scabs) are preferred for testing, but blood, tonsillar swabs, and biopsy specimens may be used.
    • Serologic studies and electron microscopy cannot distinguish between the variola virus and other orthopoxviruses.
    • Polymerase chain reaction (PCR) assays can distinguish variola virus from other orthopoxviruses.
    • Variola virus can be cultured.

Differential Diagnosis


  • Multiple rash illnesses, including the following, can be confused with smallpox:
    • Varicella and herpes zoster
    • Herpes simplex virus
    • Measles
    • Rubella
    • Monkeypox, cowpox, and tanapox
    • Viral exanthema including enterovirus
    • Disseminated molluscum contagiosum
    • Impetigo, insect bites, or scabies
    • Post-smallpox vaccine rash (vaccinia)
    • Secondary syphilis
    • Acne and contact dermatitis
    • Drug reactions including erythema multiforme
    • Meningococcemia can be confused with hemorrhagic smallpox.

Alert
  • Varicella can be confused with smallpox.
  • Varicella lesions present in different stages, are superficial, and concentrate on the trunk and face, sparing the palms and soles.
  • Smallpox lesions all present in the same stage, are deep, and concentrated on the face and limbs, often involving the palms and soles.

Treatment


Medication


  • Patients suspected of having smallpox should be vaccinated against smallpox, especially if they are in the early stages of the disease.
  • The efficacy of antiviral drugs are not known; however, cidofovir has shown efficacy in reducing smallpox virus replication in vitro and in animal studies, and tecovirimat (ST-246) shows promise and is currently in clinical trials.
  • The use of vaccinia immune globulin (VIG) can be considered for complications from vaccinia immunization but not for smallpox therapy or postexposure prophylaxis.
  • Cidofovir or VIG are available through the Strategic National Stockpile.

Additional Treatment


General Measures
  • Suspected cases of smallpox require notification of state and local authorities, who should then notify the CDC.
  • For patients with acute, generalized vesicular or pustular rash, institute airborne and contact precautions and alert infection control.
    • If high risk, report to state and local public health agency immediately.
  • Individuals recently exposed (within 3 " “4 days) to someone with contagious smallpox (e.g., someone with oral or skin lesions) should receive postexposure vaccination, as this offers the potential to limit disease and also provides significant protection from death.
  • Individuals with smallpox may be contagious during the febrile prodrome, are contagious during the early rash phase, and remain contagious until all the scabs have fallen off.

Ongoing Care


Prognosis


  • The mortality rate for variola minor was <1%.
  • Historically, the overall mortality rate for variola major was 30% but was close to 100% for the flat and hemorrhagic forms of the disease.
  • The highest mortality rates occurred among young children, pregnant women, elderly individuals, and those with immunodeficiency.
  • Long-term sequelae include pockmarks, vision loss, and limb deformities.

Complications


  • Dehydration and electrolyte abnormalities can occur during the vesicular and pustular stages and should be corrected.
  • Secondary bacterial superinfections may require antibiotic treatment.
  • Corneal ulcers or keratitis, arthritis, or encephalitis may develop.

Additional Reading


  • Besser ‚  JM, Crouch ‚  NA, Sullivan ‚  M. Laboratory diagnosis to differentiate smallpox, vaccinia, and other vesicular/pustular illnesses. J Lab Clin Med.  2003;142(4):246 " “251. ‚  [View Abstract]
  • Breman ‚  JG, Henderson ‚  DA. Diagnosis and management of smallpox. N Engl J Med.  2002;346(17):1300 " “1308. ‚  [View Abstract]
  • Moore ‚  ZS, Seward ‚  JF, Lane ‚  JM. Smallpox. Lancet.  2006;367(9508):425 " “435. ‚  [View Abstract]

Codes


ICD09


  • 050.9 Smallpox, unspecified
  • 050.0 Variola major
  • 050.2 Modified smallpox

ICD10


  • B03 Smallpox

SNOMED


  • 67924001 Smallpox (disorder)
  • 47452006 Variola major (disorder)
  • 51423006 Modified smallpox (disorder)
  • 86497005 Flat-type smallpox (disorder)
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