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Anemia, Sickle Cell

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  • Sequestration crises and hand-foot syndrome seen typically in infants/young children

  • Strokes occur mainly in childhood.

  • Adolescence/young adulthood

    • Frequency of complications and organ/tissue damage increases with age

    • Psychological complications: body image, interrupted schooling, restriction of activities; stigma of disease; low self-esteem

 
Pregnancy Considerations

  • Complicated, especially during 3rd trimester and delivery

    • Fetal mortality 35-40%. Fetal survival is >90% if the fetus reaches the 3rd trimester.

    • High prevalence of small for gestational age (SGA) babies

  • Increased risk of thrombosis, preterm delivery, pain, toxemia, infection, pulmonary infarction, phlebitis

  • Partial exchange transfusion in 3rd trimester may reduce maternal morbidity and fetal mortality, but this is controversial.

  • Chronic transfusions have been effective in diminishing pain episodes in pregnant women. However, this method should be used with caution due to risk of alloimmunization.

 

EPIDEMIOLOGY


Prevalence
  • ~90,000 Americans have sickle cell anemia (SCA) and 3.5 million people in the U.S. have sickle cell trait.
  • The condition affects mainly people of African descent. Hispanic, Middle-eastern, and Asian Indian ancestry may also be affected.

ETIOLOGY AND PATHOPHYSIOLOGY


  • Hemoglobin S results from the substitution of the amino acid valine for glutamic acid at the sixth position of the β-globin chain
  • HbS polymerizes in the RBC in the deoxygenated state resulting in RBC sickling.
  • Sickle RBCs are inflexible, causes increased blood viscosity, stasis, obstruction of small arterioles and capillaries, and ischemia.
  • Chronic anemia; crises
    • Vaso-occlusive crisis: tissue ischemia and necrosis; progressive organ failure/tissue damage from repeated episodes
    • Hand-foot syndrome: Vessel occlusion/ischemia affects small blood vessels in hands or feet.
    • Aplastic crisis: suppression of RBC production by severe infection (e.g., parvoviral and other viral infections)
    • Suppression of RBC production
    • Hyperhemolytic crisis: accelerated hemolysis with reticulocytosis; increased RBC fragility/shortened lifespan
    • Sequestration crisis: splenic sequestration of blood (only in young children as spleen is later lost to autoinfarction)
  • Susceptibility to infection: impaired/absent splenic function leading to decreased ability to clear infection; defect in alternate pathway of complement activation.
  • Increased RBC destruction causes decreased hemoglobin levels and results in anemia and fatigue.
  • Sickle cells exhibit increased adhesion and decreased ability to maneuver through small vessels, leading to vaso-occlusion.

Genetics
  • Autosomal recessive. Homozygous condition, Hb SS; heterozygous condition, Hb A/S
  • The heterozygote condition can also be combined with other hemoglobinopathies: sickle-hemoglobin C disease (HbSC) and Sβ+ thalassemia are clinically similar to the heterozygous condition, whereas, Sβ thalassemia is clinically similar to the homozygous condition.

RISK FACTORS


  • Vaso-occlusive crisis ("painful crisis"): hypoxia, dehydration, fever, infection, acidosis, cold, anesthesia, strenuous physical exercise, smoking
  • Aplastic crisis (suppression of RBC production): severe infections, human parvovirus B19 infection, folic acid deficiency.
  • Hyperhemolytic crisis (accelerated hemolysis with reticulocytosis): acute bacterial infections, exposure to oxidant

GENERAL PREVENTION


  • Prevention of crises
    • Avoid hypoxia, dehydration, cold, infection, fever, acidosis, and anesthesia.
    • Prompt management of fever, infections, pain
    • Hydration
    • Avoid alcohol and smoking.
    • Avoid high-altitude areas.
  • Minimizing trauma: aseptic technique is imperative.

DIAGNOSIS


Diagnosis is often made by newborn screening programs.  

HISTORY


  • Often asymptomatic in early months of life due to presence of fetal hemoglobin
  • In those >6 months of age, earliest symptoms are irritability and painful swelling of the hands and feet (hand-foot syndrome). May also see pneumococcal sepsis or meningitis, severe anemia and acute splenic enlargement (splenic sequestration), acute chest syndrome, pallor, jaundice, or splenomegaly.
  • Manifestations in older children include anemia, severe or recurrent musculoskeletal or abdominal pain, aplastic crisis, acute chest syndrome, splenomegaly or splenic sequestration, and cholelithiasis.
  • Painful crises in bones, joints, abdomen, back, and viscera account for 90% of all hospital admissions.
  • Acute chest syndrome: tachycardia, fever, bilateral infiltrates caused by pulmonary infarctions

PHYSICAL EXAM


Fever, pale skin and nail beds, mild jaundice  

DIFFERENTIAL DIAGNOSIS


Anemia: other hemoglobinopathies  

DIAGNOSTIC TESTS & INTERPRETATION


Initial Tests (lab, imaging)
  • Screening test: Sickledex test/Hb electrophoresis (diagnostic test of choice); SCA (FS pattern)
    • 80-100% Hb S, variable amounts of Hb F, and no Hb A1
    • Sickle cell trait (FS pattern): 30-45% Hb S, 50-70% Hb A1, minimal Hb F
  • Hemoglobin ~5 to 10 g/dL; RBC indices: mean corpuscular volume (MCV) normal to increased; mean corpuscular hemoglobin concentration (MCHC) increased; reticulocytes 3-15%
  • Leukocytosis; bands in absence of infection, platelets elevated; peripheral smear: sickled RBCs, nucleated RBCs, Howell-Jolly bodies
  • Serum bilirubin mildly elevated (2 to 4 mg/dL); ferritin very elevated in multiply transfused patients; serum lactate dehydrogenase (LDH) elevated
  • Fecal/urinary urobilinogen high
  • Haptoglobin absent or very low
  • Urine analysis: hemoglobinuria, hematuria (sickle cell trait may have painless hematuria), increased albuminuria (monitor for progressive kidney disease)
  • Need for imaging depends on clinical circumstances
    • Bone scan to rule out osteomyelitis
    • CT/MRI to rule out CVA; high index of suspicion required for any acute neurologic symptoms other than mild headache.
    • Chest x-ray: may show enlarged heart; diffuse alveolar infiltrates in acute chest syndrome
    • Transcranial Doppler: start at age 2 years; repeat yearly. Transcranial Doppler ultrasound identifies children age 2 to 16 years at higher risk of stroke.
    • ECG to detect pulmonary hypertension and echocardiogram every other year from age 15 years and older.

Test Interpretation
Hyposplenism due to autosplenectomy is common; hypoxia/infarction in multiple organs  

TREATMENT


GENERAL MEASURES


  • Painful crises: hydration, analgesics; oxygen regardless of whether the patient is hypoxic (1)[A]
  • Retinal evaluation starting at school age to detect proliferative sickle retinopathy (1,2)[A]
  • Occupational therapy, cognitive and behavioral therapies, support groups
  • All standard childhood vaccinations should be administered accordingly.
  • Special immunizations
    • Influenza vaccine yearly
    • Conjugated pneumococcal vaccine (PCV13) at ages 2, 4, and 6 months; booster at 12 to 15 months.
    • Patients <5 years of age with incomplete vaccination history should receive catch-up doses accordingly.
    • Adults age ≥19 years who have functional asplenia and have not received pneumococcal vaccination should receive one dose PCV13, followed by administration of PPSV23 at least 8 weeks later (2)[A].
  • Meningococcal vaccine:
    • 6 weeks old: Hib-MenCY at ages 2, 4, 6, and 12 months
    • 9 months old: two doses of MCV4 separated by 3 months
    • ≥2 years of age: two doses of MCV4-D-CRM separated by 2 months; boosters recommended every 5 years

MEDICATION


First Line
  • Prophylactic penicillins indicated in infants and children starting at 2 months: A dose of 125 mg BID is recommended for children <5 years. A dose of 250 mg BID is recommended for children >5 years. Amoxicillin 20 mg/kg/day is an alternative to penicillin; if high risk remains, continue until puberty. Rising pneumococcal resistance to penicillin may change future recommendations (3)[A].
  • Supplemental oxygen
  • Painful crises (mild, outpatient)
    • Nonnopioid analgesics (ibuprofen)
  • Painful crises (severe, hospitalized)
    • Parenteral opioids (e.g., morphine on fixed schedule); patient-controlled analgesia (PCA) pump may be useful (2)[A].
  • Hydroxyurea for prevention of painful acute chest syndrome, vaso-occlusive episodes, and very severe anemia. Increases fetal hemoglobin concentration. Adults: Start with 15 mg/kg/day single daily dose; Children: 20 mg/kd/day; titrate upward every 8 weeks (max dose of 35 mg/kg/day) Monitor blood counts satisfactory (avoid severe neutropenia, thrombocytopenia) (2)[A].
  • Acute chest syndrome: may deteriorate quickly; monitor patients with vaso-occlusive crisis with incentive spirometry. Treat with aggressive management with oxygen, analgesics, antibiotics, simple or exchange transfusion (2)[A].
  • Empiric antibiotics to cover Mycoplasma pneumoniae, and Chlamydia pneumoniae (cephalosporins or azithromycin) (1)[A]. If osteomyelitis, cover for Staphylococcus aureus and Salmonella (e.g., ciprofloxacin). Precautions: Avoid high-dose estrogen oral contraceptives; consider Depo-Provera. G-CSF use is contraindicated as it may lead to vaso-occlusive episodes and multiorgan failure.

Second Line
Folic acid: 0 to 6 months: 0.1 mg/day; 6 to 12 months: 0.25 mg/day; 1 to 2 years: 0.5 mg/day; >2 years of age: 1 mg/day  

ADDITIONAL TREATMENT


Transfusions and additional therapies (4)[A]  
  • Transfusion for aplastic crises, severe complications (i.e., CVA), prophylactically before surgery, and treatment for acute chest syndrome; prophylactic transfusions for primary or secondary stroke prevention in children
  • Preoperative transfusions have been shown to reduce the risk of perioperative complications.
  • Avoid blood hyperviscosity.
  • Consider chelation with deferasirox, an oral agent, if the patient is multiply transfused (after age 2 years). Red cell exchange transfusion minimizes risk of iron overload.

SURGERY/OTHER PROCEDURES


  • Targeted fetal hemoglobin induction treatment (5)[A]
  • Hematopoietic stem cell transplant (HSCT) (6)[A]: curative, but with significant morbidity and mortality; limited to individuals <16 years old.

INPATIENT CONSIDERATIONS


Admission Criteria/Initial Stabilization
Severe pain, suspected infection or sepsis, evidence of acute chest syndrome  
IV Fluids
The preferred maintenance IV fluid is ˝; NS, as NS may theoretically increase the risk of sickling.  

ONGOING CARE


FOLLOW-UP RECOMMENDATIONS


Patient Monitoring
  • Treat infections early. Parents/patients: Any temperature of ≥101 °F (38.3 °C) requires immediate medical attention.
  • For patients who receive chronic transfusions, monitor for hepatitis C and hemosiderosis.
  • Periodic eye evaluations: starting age 10 years to detect proliferative sickle retinopathy; re-screen 1 to 2 year intervals (3)[A]
  • Biannual examination for hepatic, renal, and pulmonary dysfunction
  • Neuroimaging screening for risk of stroke: Transcranial Doppler beginning at age 2 years and continuing up to age 16 years
  • Baseline pulmonary evaluation at each visit to assess for wheezing, shortness of breath, or cough (indicators of disease severity and pulmonary hypertension). Echocardiography for symptomatic patients; right heart catheterization for diagnosis
  • Consider venous thromboembolism (VTE) prophylaxis as there is an increased incidence of thromboembolism.

DIET


  • Folic acid supplementation; avoid alcohol (leads to dehydration); maintain hydration
  • Multivitamin without iron is recommended as there is high incidence of vitamin D deficiency and decreased bone marrow density in SCD patients.

PATIENT EDUCATION


  • SickleCellKids.org-Education Web site for children with sickle cell anemia: http://www.sicklecelldisease.org
  • American Sickle Cell Anemia Association: http://www.ascaa.org

PROGNOSIS


  • Anemia occurs in infancy; sickle cell crises at 1 to 2 years of age; some children die in their first year.
  • In adulthood, fewer crises, but more complications. Median age of death is 42 years for men and 48 years for women. Complications are mentioned below.

COMPLICATIONS


  • Alloimmunization, bone infarct and osteomyelitis, aseptic necrosis of femoral head
  • CVA (peak age 6 to 7 years), impaired mental development, even without history of stroke
  • Cholelithiasis/abnormal liver function
  • Chronic leg ulcers, poor wound healing
  • Impotence, priapism, hematuria/hyposthenuria, renal complications (proteinuria)
  • Retinopathy, splenic infarction (by age 10 years)
  • Acute chest syndrome (infection/infarction) leading to chronic pulmonary disease
  • Infections (pneumonia, osteomyelitis, meningitis, pyelonephritis); sepsis (leading cause of morbidity and mortality)
  • Hemosiderosis (secondary to multiple transfusions)
  • Substance abuse related to chronic opioid use

REFERENCES


11 Brousse  V, Makani  J, Rees  DC. Management of sickle cell disease in the community. BMJ.  2014;348:g1765.22 Yawn  BP, Buchanan  GR, Afenyi-Annan  AN, et al. Management of sickle cell disease: summary of the 2014 evidence-based report by expert panel members. JAMA.  2014; 312 (10): 1033-1048.33 Cober  MP, Phelps  SJ. Penicillin prophylaxis in children with sickle cell disease. J Pediatr Pharmacol Ther.  2010;15(3):152-159.44 Chou  ST. Transfusion therapy for sickle cell disease: a balancing act. Hematology Am Soc Hematol Educ Program.  2013;2013:439-446.55 Manwani  D, Frenette  PS. Vaso-occlusion in sickle cell disease: pathophysiology and novel targeted therapies. Blood.  2013;122(24):3892-3898.66 Hsieh  MM, Fitzhugh  CD, Weitzel  RP, et al. Nonmyeloablative HLA-matched sibling allogeneic hematopoietic stem cell transplantation for severe sickle cell phenotype. JAMA.  2014;312(1):48-56.

ADDITIONAL READING


  • Aragona  E, Kelly  MJ. Hyperhemolysis in sickle cell disease. J Pedriatr Hematol Oncol.  2014;36(1):354-356. doi:10/1097/MPH.0b013e31828e529f.
  • Costa  VM, Viana  MB, Aguilar  RA. Pregnancy in patients with sickle cell disease: maternal and perinatal outcomes. J Matern Fetal Neonatal Med.  2015;28(6):685-689. doi:10.3109/14767058.2014.928855.
  • Machado  RF, Farber  HW. Pulmonary hypertension associated with chronic hemolytic anemia and other blood disorders. Clin Chest Med.  2013;34(4):739-752.
  • Naik  RP, Streiff  MB, Lanzkron  S. Sickle cell disease and venous thromboembolism: what the anticoagulation expert needs to know. J Thromb Thrombolysis.  2013;35(3):352-358.

SEE ALSO


Algorithm: Anemia  

CODES


ICD10


  • D57.1 Sickle-cell disease without crisis
  • D57.3 Sickle-cell trait
  • D57.00 Hb-SS disease with crisis, unspecified
  • D57.01 Hb-SS disease with acute chest syndrome
  • D57.20 Sickle-cell/Hb-C disease without crisis
  • D57.212 Sickle-cell/Hb-C disease with splenic sequestration
  • D57.219 Sickle-cell/Hb-C disease with crisis, unspecified
  • D57.02 Hb-SS disease with splenic sequestration
  • D57.80 Other sickle-cell disorders without crisis
  • D57.412 Sickle-cell thalassemia with splenic sequestration
  • D57.811 Other sickle-cell disorders with acute chest syndrome
  • D57.819 Other sickle-cell disorders with crisis, unspecified
  • D57.812 Other sickle-cell disorders with splenic sequestration
  • D57.411 Sickle-cell thalassemia with acute chest syndrome
  • D57.40 Sickle-cell thalassemia without crisis
  • D57.211 Sickle-cell/Hb-C disease with acute chest syndrome
  • D57.419 Sickle-cell thalassemia with crisis, unspecified

ICD9


  • 282.60 Sickle-cell disease, unspecified
  • 282.5 Sickle-cell trait
  • 282.61 Hb-SS disease without crisis
  • 282.62 Hb-SS disease with crisis
  • 282.64 Sickle-cell/Hb-C disease with crisis
  • 282.68 Other sickle-cell disease without crisis
  • 282.69 Other sickle-cell disease with crisis
  • 282.63 Sickle-cell/Hb-C disease without crisis

SNOMED


  • 127040003 Hereditary hemoglobinopathy disorder homozygous for hemoglobin S (disorder)
  • 16402000 Sickle cell trait (disorder)
  • 416180004 Hemoglobin SS disease without crisis
  • 417425009 Hemoglobin SS disease with crisis (disorder)
  • 417517009 Sickle cell-hemoglobin C disease with crisis
  • 417683006 Sickle cell-hemoglobin C disease without crisis

CLINICAL PEARLS


  • Over 90,000 Americans have SCA (~1 in 375 African Americans).The preferred maintenance IV fluid is 1/2 NS, as NS may theoretically increase the risk of sickling.
  • Painful crises in bones, joints, abdomen, back, and viscera account for 90% of all hospital admissions.
  • Acute chest syndrome: tachycardia, fever, bilateral infiltrates caused by pulmonary infarctions
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