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Lymphoma, Burkitt

para>Common age group (30% of cases in the United States) ‚  
Geriatric Considerations

Unusual in this age group. Toxicity with chemotherapy may be increased in the elderly.

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Pregnancy Considerations

With aggressive treatment, good maternal, and fetal outcome

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EPIDEMIOLOGY


  • Varies by disease form
  • Endemic
    • One of most common tumors of childhood in Africa, most frequently occurring in children age 4 to 7 years
    • Rare in adults
  • Sporadic
  • In the United States, trimodal peaks of age incidence around ages 10, 40, and 75 years
  • More common in Caucasians
  • Predominant sex: male > female (3:1 or 4:1)

Incidence
Rare in the United States, incidence 0.27 per 100,000 person-years; 50 times more common in endemic regions of Africa ‚  
Prevalence
Composes <1% of adult non-Hodgkin lymphoma (NHL); accounts for 30 " “40% of NHL in children in the United States and Western Europe ‚  

ETIOLOGY AND PATHOPHYSIOLOGY


  • Activation and overexpression of c-Myc oncogene (1)
  • Monoclonal proliferation of B lymphocytes resulting from dysregulation of c-Myc
    • Translocation of c-Myc to immunoglobulin coding regions results in constitutive expression of gene product.
    • EBV-infected cells in germinal center reactions may increase the risk of translocation.
  • Poorly regulated proliferation of genetically unstable B cells increases chance of translocations:
    • Immunodeficient patients with persistent generalized lymphadenopathy and polyclonal B-cell activation

RISK FACTORS


Pediatric Considerations

Endemic: Children with early acquisition of EBV infection are at increased risk. Coinfection with malaria and EBV increases incidence 100-fold.

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GENERAL PREVENTION


No known methods to prevent Burkitt lymphoma ‚  

COMMONLY ASSOCIATED CONDITIONS


  • EBV infection
  • Immunodeficiency, especially AIDS

DIAGNOSIS


HISTORY


  • Rapidly progressive bulky adenopathy or extranodal mass
  • Symptoms of bone marrow involvement
    • Fatigue, exercise intolerance, bruising, epistaxis, other bleeding, fever
  • Abdominal presentation
    • Abdominal pain, nausea, vomiting, bowel obstruction, GI bleeding, symptoms mimicking acute appendicitis or intussusception
  • Endemic (African): jaw or facial bone tumor, with mouth pain, loose teeth, or jaw mass
  • Nonendemic: extranodal disease, abdominal presentation typical
  • Can present as acute leukemia (L3-ALL) with predominant bone marrow involvement and no mass lesions
  • Renal function impairment and significant metabolic derangement may quickly manifest due to the rapid progression and spread of the tumor.

PHYSICAL EXAM


  • Endemic: mass on jaw or facial bone, pallor, petechiae, hepatosplenomegaly
  • Sporadic: lymphadenopathy, any mass lesion, abdominal tenderness, pallor, petechiae, hepatosplenomegaly

DIFFERENTIAL DIAGNOSIS


  • Other NHLs
    • Burkitt-like lymphoma: intermediate immunophenotype and molecular characteristics between classic Burkitt lymphoma and DLBCL
    • DLBCL: large, irregular cells, often with B-cell lymphoma (BCL) rearrangement
    • Precursor B-lymphoblastic lymphoma
    • Precursor T-lymphoblastic lymphoma
    • Mantle cell lymphoma, blastoid variant
  • Hodgkin lymphoma
  • Acute lymphoblastic leukemia
  • Other causes of lymphadenopathy
    • Infection (e.g., bacterial lymphadenitis, mononucleosis, tuberculosis, atypical mycobacterium, cat-scratch disease)
    • Reactive lymphoid hyperplasia
    • Histiocytosis
  • Other primary malignancies of childhood (e.g., Wilms tumor, neuroblastoma, peripheral neuroectodermal tumor)
  • Other metastatic malignancies

DIAGNOSTIC TESTS & INTERPRETATION


Initial Tests (lab, imaging)
  • Biopsy of mass lesion: diagnosis by cellular morphology on histologic examination
  • CBC with differential: anemia, neutropenia, or thrombocytopenia
  • Tumor lysis syndrome: hyperkalemia, hyperphosphatemia, hyperuricemia, renal failure. Order electrolytes, BUN, creatinine, calcium, magnesium, phosphorus, serum lactate dehydrogenase (LDH), uric acid.
  • Consider hepatitis B virus serologies prior to rituximab
  • Chest x-ray
  • CT scan of chest, abdomen, pelvis
  • Consider whole body PET to identify active disease.
  • Dedicated imaging of any site suspected to be involved by tumor

Follow-Up Tests & Special Considerations
  • Diagnosis requires immunophenotypic and cytogenetic data.
  • Immunophenotype studies
    • Cells express surface immunoglobulin (Ig) M- and B-associated antigens (CD19, CD20, CD22, CD79a), as well as CD10, HLA-DR, and CD43.
    • Cells also show nuclear staining for BCL-6 protein.
  • Cytogenic studies to visualize chromosomal translocation
    • Reciprocal chromosome translocation involving c-Myc and immunoglobulin heavy chain (IgH) gene (t[8;14]) (80% of cases) or immunoglobulin light chain (IgL) genes (t[2;8] or t[8;22])
    • Fluorescence in situ hybridization (FISH) or long-segment polymerase chain reaction (PCR) may be necessary to identify translocation.
  • EBV testing in lesional cells
  • Gene expression profiling can help distinguish Burkitt lymphoma from DLBCL.

Diagnostic Procedures/Other
  • Bone marrow aspiration and biopsy for morphology and flow cytometry
  • Lumbar puncture for CSF cell count, differential, and cytology
  • Lymph node biopsy: Most lymph nodes suggestive of disease should be selected for excisional biopsy.
    • Frozen sections and needle biopsies discouraged because lymph node architecture helpful for diagnosis.
  • Diagnostic laparotomy with resection of localized disease

Test Interpretation
  • Monotonous diffuse infiltrate of medium-sized round cells, with round or oval nuclei, several nucleoli, and coarse chromatin. Cytoplasm is intensely basophilic and moderately abundant.
  • Mitotic rate is high; close to 100% of viable cells will be actively engaged in cell cycle.
  • Classic starry-sky histologic appearance
    • Results from the presence of scattered macrophages with phagocytic cell debris
    • Characteristic of, although not pathognomonic for, Burkitt lymphoma

TREATMENT


If available, all patients should be offered participation in an appropriate clinical trial. ‚  

MEDICATION


First Line
  • Intensive, short-term, multiagent chemotherapy administered in cycles (2)[A]
    • Chemotherapeutic agents include cyclophosphamide, methotrexate, vincristine, prednisone, high-dose methotrexate, high-dose cytarabine, etoposide, ifosfamide, and doxorubicin.
    • Type and extent of therapy depend on stage of disease.
  • Rituximab in combination with chemotherapy may improve outcome (3)[B].
  • Nonintensive chemotherapy protocols used in developing countries can be effective (4)[B].
    • Cyclophosphamide, methotrexate
    • Areas with limited financial and medical resources
  • CNS prophylaxis for most patients
    • Not necessary for limited disease far from CNS
    • Intrathecal methotrexate, cytarabine, and hydrocortisone, with or without IV methotrexate and cytarabine, may be used for CNS prophylaxis.
    • Prophylactic irradiation does not improve outcome.
  • Chemotherapy cycles should be initiated as soon as hematologic recovery permits.
    • Delay of chemotherapy may result in regrowth of resistant tumor between cycles.
  • Management of tumor lysis syndrome with initial cycle of chemotherapy

Second Line
  • Rituximab may be effective if not used previously.
  • Hematopoietic stem cell transplantation in combination with high-dose chemotherapy (5,6)[B]

ISSUES FOR REFERRAL


All patients should be treated by a pediatric or adult oncologist. ‚  

SURGERY/OTHER PROCEDURES


  • Biopsy and staging
    • All patients require a biopsy to establish the diagnosis pathologically.
  • Surgical resection
    • Treatment for small, completely resectable abdominal tumors (in addition to chemotherapy)
    • For patients with intestinal obstruction who cannot begin chemotherapy immediately
    • Aggressive upfront resection of disease is not indicated.
  • Patients require placement of a central venous line for administration of chemotherapy.

COMPLEMENTARY & ALTERNATIVE MEDICINE


Many complementary therapies exist to assist in management of side effects of chemotherapy. ‚  

INPATIENT CONSIDERATIONS


Admission Criteria/Initial Stabilization
  • Almost all patients should be admitted to the hospital for initial care.
  • Burkitt lymphoma has high-growth fraction and short doubling time.
    • Rapid initiation of definitive chemotherapy is essential.
  • Management of tumor lysis syndrome with initial cycle of chemotherapy
    • Aggressive hydration without potassium
    • Close monitoring of electrolytes, renal function, and uric acid initially q6 " “8h.
    • Rasburicase (0.2 mg/kg IV once daily for up to 5 days, depending on response to therapy) to break down uric acid
    • Allopurinol (10 mg/kg PO divided 2 to 3/day) if rasburicase not available
      • Consider alkalization of urine with bicarbonate-containing IV fluids(goal urine pH 7 to 8) when using allopurinol.
    • Phosphate binder if serum phosphorus becomes elevated
    • Avoid supplemental calcium unless symptomatic hypocalcium develops.
    • Medical management of hyperkalemia

IV Fluids
Aggressive IV hydration with first cycle of chemotherapy ‚  
  • Typically D5/0.5 NS at 125 mL/m2/hr (twice the maintenance rate)
  • No potassium in IV fluids

ONGOING CARE


FOLLOW-UP RECOMMENDATIONS


Patient Monitoring
  • Close monitoring of serum chemistries is critical due to high risk of tumor lysis syndrome and uric acid nephropathy.
  • CBC, liver function, and renal function should also be closely monitored throughout chemotherapy.
  • Surveillance history and physical exam for detection of recurrence
  • Consideration of routine imaging for detection of recurrence (7)[B]
  • All patients, particularly children, should be followed indefinitely for long-term effects of chemotherapy.

PATIENT EDUCATION


Educational materials are available online from ‚  
  • Leukemia and Lymphoma Society (www.leukemia-lymphoma.org)
  • CureSearch (www.curesearch.org)

PROGNOSIS


  • Low-stage localized disease, 5-year event-free survival >95%
  • Aggressive treatment of advanced disease yields >90% 5-year event-free survival (8).
  • Additional chromosomal abnormalities may be associated with poorer prognosis (9).
  • Recurrent disease more resistant to therapy
  • Mortality for endemic form remains high where access to health care is limited.

COMPLICATIONS


  • Complications of extensive abdominal disease include obstructive jaundice and pancreatitis, bowel obstruction, and intestinal perforation.
  • Tumor lysis syndrome with renal failure (uric acid nephropathy) may occur prior to, and especially following, the start of chemotherapy.
  • Rituximab has been associated with reactivation of hepatitis B virus resulting in fulminant liver failure.
  • Other effects of chemotherapy include alopecia, myelosuppression, life-threatening infection, nausea, mucositis, infusion reactions, peripheral neuropathy, seizures, infertility, congestive heart failure, and secondary malignancy.

REFERENCES


11 Said ‚  J, Lones ‚  M, Yea ‚  S. Burkitt lymphoma and MYC: what else is new? Adv Anat Pathol.  2014;21(3):160 " “165.22 Okebe ‚  JU, Skoetz ‚  N, Meremikwu ‚  MM, et al. Therapeutic interventions for Burkitt lymphoma in children. Cochrane Database Syst Rev.  2011;(7):CD005198.33 Rizzieri ‚  DA, Johnson ‚  JL, Byrd ‚  JC, et al. Improved efficacy using rituximab and brief duration, high intensity chemotherapy with filgrastim support for Burkitt or aggressive lymphomas: cancer and leukemia Group B study 10 002. Br J Haematol.  2014;165(1):102 " “111.44 Beogo ‚  R, Nacro ‚  B, Ouedraogo ‚  D, et al. Endemic Burkitt lymphoma of maxillofacial region: results of induction treatment with cyclophosphamide plus methotrexate in West Africa. Pediatr Blood Cancer.  2011;56(7):1068 " “1070.55 Gross ‚  TG, Hale ‚  GA, He ‚  W, et al. Hematopoietic stem cell transplantation for refractory or recurrent non-Hodgkin lymphoma in children and adolescents. Biol Blood Marrow Transplant.  2010;16(2):223 " “230.66 Maramattom ‚  LV, Hari ‚  PN, Burns ‚  LJ, et al. Autologous and allogeneic transplantation for burkitt lymphoma outcomes and changes in utilization: a report from the center for international blood and marrow transplant research. Biol Blood Marrow Transplant.  2013;19(2):173 " “179.77 Eissa ‚  HM, Allen ‚  CE, Kamdar ‚  K, et al. Pediatric Burkitt 's lymphoma and diffuse B-cell lymphoma: are surveillance scans required? Pediatr Hematol Oncol.  2014;31(3):253 " “257.88 El-Mallawany ‚  NK, Cairo ‚  MS. Advances in the diagnosis and treatment of childhood and adolescent B-cell non-Hodgkin lymphoma. Clin Adv Hematol Oncol.  2015;13(2):113 " “123.99 Lones ‚  MA, Sanger ‚  WG, Le Beau ‚  MM, et al. Chromosome abnormalities may correlate with prognosis in Burkitt/Burkitt-like lymphomas of children and adolescents: a report from Children 's Cancer Group Study CCG-E08. J Pediatr Hematol Oncol.  2014;26(3):169 " “178.

CODES


ICD10


  • C83.70 Burkitt lymphoma, unspecified site
  • C83.71 Burkitt lymphoma, lymph nodes of head, face, and neck
  • C83.73 Burkitt lymphoma, intra-abdominal lymph nodes
  • C83.79 Burkitt lymphoma, extranodal and solid organ sites
  • C83.77 Burkitt lymphoma, spleen
  • C83.76 Burkitt lymphoma, intrapelvic lymph nodes
  • C83.74 Burkitt lymphoma, lymph nodes of axilla and upper limb
  • C83.72 Burkitt lymphoma, intrathoracic lymph nodes
  • C83.75 Burkitt lymphoma, nodes of inguinal region and lower limb
  • C83.78 Burkitt lymphoma, lymph nodes of multiple sites

ICD9


  • 200.20 Burkitt 's tumor or lymphoma, unspecified site, extranodal and solid organ sites
  • 200.21 Burkitt 's tumor or lymphoma, lymph nodes of head, face, and neck
  • 200.23 Burkitt 's tumor or lymphoma, intra-abdominal lymph nodes
  • 200.28 Burkitt 's tumor or lymphoma, lymph nodes of multiple sites
  • 200.27 Burkitt 's tumor or lymphoma, spleen
  • 200.26 Burkitt 's tumor or lymphoma, intrapelvic lymph nodes
  • 200.24 Burkitt 's tumor or lymphoma, lymph nodes of axilla and upper limb
  • 200.22 Burkitt 's tumor or lymphoma, intrathoracic lymph nodes
  • 200.25 Burkitt 's tumor or lymphoma, lymph nodes of inguinal region and lower limb

SNOMED


  • Burkitt 's lymphoma - disorder
  • Burkitt 's tumor of lymph nodes of head, face AND/OR neck
  • Burkitt 's tumor of intra-abdominal lymph nodes
  • Burkitt 's tumor of lymph nodes of multiple sites
  • Burkitt 's tumor of lymph nodes of inguinal region AND/OR lower limb
  • Burkitt 's tumor of intrathoracic lymph nodes
  • Burkitt 's tumor of lymph nodes of axilla AND/OR upper limb
  • Burkitt 's tumor of spleen
  • Burkitt 's tumor of intrapelvic lymph nodes

CLINICAL PEARLS


  • Burkitt lymphoma is an aggressive, mature B-cell malignancy most commonly diagnosed in childhood.
  • Burkitt lymphoma is strongly associated with t(8;14) and with EBV and malaria infections.
  • Burkitt lymphoma is highly treatable with intense multiagent systemic and intrathecal chemotherapy.
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