This test is used for the diagnosis of enteric infections caused by Rotavirus.
This test may be ordered for patients, usually young children, who present with a sudden onset of watery diarrhea, which is often preceded by vomiting.
Unpreserved stool is submitted for testing.
Rotavirus antigen in stool is detected by immunologic techniques using rotavirus-specific antibodies. LA and EIA formats are typically used. Sensitivity and specificity of commercial EIA assays are reported in the high 90% range.
Turnaround time: <24 hours.
Interpretation
Expected results: Negative.
Limitations
LA tests have reported sensitivities less than the sensitivities of EIA assays. Assays may be less reliable in neonates.